Identification and characterisation of seed storage protein transcripts from Lupinus angustifolius
نویسندگان
چکیده
منابع مشابه
Precursors of storage proteins in Lupinus angustifolius.
The proteins that are synthesized during differentiation and development in the cotyledons of Lupinus angustifolius L. were characterized both in situ and after purification. The proteins present in situ were separated by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and subjected to 'Western'-blot analysis to identify immunologically related polypeptides. The major storage protein...
متن کاملSeed Hydropriming and Smoke Water Significantly Improve Low-Temperature Germination of Lupinus angustifolius L.
Seed imbibition under cold temperature is dangerous when dry seeds have relatively low water content. The aim of this study was to investigate germination of 20 lines/cultivars of narrow-leaf lupine at 7 °C (cold) and 13 °C (control) under the influence of smoke water and following seed hydropriming for 3 h at 20 °C. The efficacy of individual treatments was examined with regard to seed protect...
متن کاملThe aspartate aminotransferase-P2 gene from Lupinus angustifolius.
and plays a key role in carbon and nitrogen metabolism in plants. The enzyme has been shown to be involved in the shuttling of reducing equivalents from the cytoplasm to chloroplasts, mitochondria, glyoxysomes, and peroxisomes via the malate-aspartate shuttle (Wightman and Forest, 1978). Up to five separate isoforms of AAT have been reported in plants, differing both kinetically and in levels a...
متن کاملThe energy value of Lupinus angustifolius and Lupinus albus for growing pigs
Ninety male crossbred pigs were allocated at 30 kg live weight to a 6 5 factorial experiment involving six diets and five levels of feeding where average daily intakes were 1.11, 1.36, 1.67, 1.90 kg and ad libitum between 30 and 60 kg live weight. The control diet contained predominantly animal protein sources, another four diets contained 350 g/kg of either kernels or seeds of either L. angust...
متن کاملPreliminary results on the effects of grape (Vitis vinifera) seed condensed tannins on in vitro intestinal digestibility of the lupin (Lupinus angustifolius) seed protein fraction in small ruminants.
Condensed tannins (CT) from grape seeds (Vitis vinifera L.) were added to complex the protein fraction of Lupinus angustifolius seeds. Three CT/protein ratios were used: 96 mg/g (T(1)), 180 mg/g (T(2)) and 0 mg/g (T(0)). The CP losses in the rumen were assessed by the nylon-bag technique and CP intestinal digestibility (CPID) was estimated using an in vitro assay applying a three-step procedure...
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ژورنال
عنوان ژورنال: BMC Plant Biology
سال: 2011
ISSN: 1471-2229
DOI: 10.1186/1471-2229-11-59